LAMP Premix (Probe) (Bst2.0 HS)

LAMP (Loop-mediated isothermal amplification) is a nucleic acid amplification technique. This assay kit utilizes probe-based isothermal amplification and can be used for qualitative detection of DNA in samples. Bst2.0, derived from Bacillus stearothermophilus DNA polymerase, is used in this kit. Compared to wild-type Bst DNA polymerase, Bst2.0 can effectively enhance amplification speed and yield. Bst2.0 possesses 5'→3' DNA polymerase activity and strong strand displacement capability, but lacks 3'→5' exonuclease activity. It can be used for DNA strand displacement reactions and LAMP amplification. Bst2.0 HS is a heat-activated DNA polymerase based on Bst2.0, obtained through reversible modification technology. It completely blocks enzyme activity at room temperature and enables reaction setup at room temperature, preventing nonspecific amplification and improving reaction efficiency. Additionally, Bst2.0 HS DNA polymerase does not require a separate activation step.

This assay kit utilizes LAMP technology and is designed with 4-6 specific LAMP primers targeting the desired sequence, as well as a probe dependent on RNase HⅡ. Bst2.0 HS is used for strand displacement and polymerase activity to amplify the target fragment. The specific RNase HⅡ recognizes the probe bound to the double-stranded DNA target sequence and cleaves the probe, emitting a fluorescence signal. The amplification can be determined by detecting the fluorescence signal using an instrument.

Specification:

Reaction Temperature: 60~65℃, 30~60 min (collect fluorescence every minute) 

Lyophilization: Yes

Composition of the Premix

1. Bst2.0 HS (8 U/µL)

2. 2×LAMP Premix Buffer II

3. RNase H II (50 mU/μL)

Recommended Application:

1. Applicable to LAMP dye detection of DNA samples, which requires an instrument to detect fluorescence.

2. Set up the reaction at room temperature to prevent non-specific amplification and improve reaction efficiency.