RT-LAMP Premix (Probe) (Bst2.0 HS)

LAMP (Loop-mediated isothermal amplification) is a nucleic acid amplification technique. This assay kit utilizes LAMP technology and is designed with 4-6 specific LAMP primers targeting the desired sequence, as well as a probe dependent on RNase H II. Bst2.0 HS is used for strand displacement and polymerase activity to amplify the target fragment. The specific RNase H II recognizes the probe bound to the double-stranded DNA target sequence and cleaves the probe, emitting a fluorescence signal. The amplification can be determined by detecting the fluorescence signal using an instrument.

Product Specification:

Reaction Temperature: 60~65℃, 30~60 min (collect fluorescence every minute) 

Lyophilization: Yes

Composition of the Premix

1. Bst2.0 HS (8 U/µL)

2. 2×RT-LAMP Premix Buffer II

3. Neoscript RTase (200 U/µL)

4. RNase H II (50 mU/μL)