LAMP Premix (DyeⅠ) (Bst2.0 HS)

LAMP (Loop-mediated isothermal amplification) is a nucleic acid amplification technique. This assay kit utilizes colorimetric-based isothermal amplification and can be used for qualitative detection of DNA in samples. Bst2.0, derived from Bacillus stearothermophilus DNA polymerase, is used in this kit. Compared to wild-type Bst DNA polymerase, Bst2.0 can effectively enhance amplification speed and yield. Bst2.0 possesses 5'→3' DNA polymerase activity and strong strand displacement capability, but lacks 3'→5' exonuclease activity. It can be used for DNA strand displacement reactions and LAMP amplification. Bst2.0 HS is a heat-activated DNA polymerase based on Bst2.0, obtained through reversible modification technology. It completely blocks enzyme activity at room temperature and enables reaction setup at room temperature, preventing nonspecific amplification and improving reaction efficiency. Additionally, Bst2.0 HS DNA polymerase does not require a separate activation step.

This kit uses Lamp technology. 6 specific LAMP primers are designed for the target sequence. BST2.0 HS isothermal amplification enzyme and specific primers are used to identify 6 independent regions of the target sequence. Double-stranded DNA produced by isothermal amplification combines with Eva Green to emit a fluorescent signal. The fluorescent signal is detected by the instrument to determine whether amplification has occurred.

Compositions:

1. 2×LAMP Premix Buffer Ⅱ

2. BST2.0 HS (8 U/µL)

3. 50×LAMP Fluorecent Dye Ⅰ

Recommended Application:

1. Applicable to LAMP dye detection of DNA samples, which requires an instrument to detect fluorescence.

2. Set up the reaction at room temperature to prevent non-specific amplification and improve reaction efficiency.